文档介绍：临床医学论文发表来源:创新医学网(乙酯对D-氨基半乳糖诱导的急性肝损伤大鼠的保护作用何灿明王鲁文任巧晶龚作炯【摘要】目的观察***酸乙酯(EP)对D-氨垒半乳糖盐酸盐诱导的急性肝损伤模型大鼠的保护作川,并探讨其作用机制。方法制备D-氨基半乳糖盐酸盐诱导的急性肝损伤大鼠模型48只,并随机分成正常组、模烈组、小剂量EP提前干预组、人剂量EP提前干预组、小剂量EP治疗组和大剂量EP治疗组。在造模后24h时取血,采用ELTSA法检测TNF-a、IFN-¥、TL-1O和TI-18水平;采用RT-PCR法检测肝组织HMGB1mRNA水平变化。结果与止常组比较,模型组人鼠血淸TNF・a、TFN-Y>TL-1O和儿-18水平均无明显变化(),而肝组织HMGB1mRNA水平明显升高(P<);与模型组比较,EP提前干预组和EP治疗组动物血清TNF-a、IFN-y、1L-10和1L-18水平均无明显变化,而EP提前干预则显著降低了肝组织HMGB1mRNA水平(),其中大剂量组最为显著();病理学检査显示,EP提前干预组大鼠肝组织学得到明显改善,尤其是在人剂量组,而且比EP治疗组更加显著。结论EP能冇效保护D■氨基半乳糖盐酸盐诱导的大鼠急性肝损伤,保护效果与药物的干预剂量成止相关,并且提前干预的效果更明显。【关键词】急性肝损伤;***酸乙酯;高迁移率族蛋白Bl;D-氨基半乳糖TheprotectiveeffectofethylpyruvateonacutehepaticinjuryinducedbyD-galactostiminehydrochlorateinratsHECanming,WANGLuwen,RENQiaojing,,People"sHospital,WuhanUniversity,Wuhan,430060,China[Abstract]ObjectiveToobservetheprotectiveeffectofethylpyruvate(EP)onacutehepaticinjuryinducoclbyD-galactosaminchydrochlorateinras・MethodsTheacutehepaticinjuryinducedbyD-galactosciminehydrochloratewerepreparedin48maleWistarratsandtheanimalsweredividedatrandomintonotmal,model,earlyinterferenceoflowdoseofEP,earlyinterfereneeofhighdoseofEP,,theserumlevelsofTNF-a,TFN-Y,IL-10andIL-18wereassayedbyELISA,thechangesofhighmobilitygroupbox1protein(HMGB1)mRNAinlivertissuesweredeterininedbyRT-PCR,andthepathologicalchan^(P<),whileserumlevelsofTNF-a,TFN-Y»TL-10andTL-18inmodelgrouphadnoobviouschanges(P>)・Comparedwithmodelgroup,thelevelsofserumTNF-a,IFN-Y,IL-10andIL-18hadnoobviouschangesinEPearlyinterferencegrouporinEPtreatmentgroup,butthe11MGB1mRNAlevelsinlivertissuesinEPearlyinterfereneegroupwereobviouslydecreased(P<),especiallyinhigh-dosegroup,whichwasmoreobviousthaninEPtreatmentgroup(P<)・Thehistopathologicalchangesinthe1iverswasmostsignificantlyimprovedinEPear