文档介绍：肺腺癌细胞对谷胱甘肽纳米Fe3O4的内吞作用本文从网络收集而来,上传到平台为了帮到更多的人,如果您需要使用本文档,请点击下载按钮下载本文档(有偿下载),另外祝您生活愉快,工作顺利,万事如意! 【关键词】人肺腺癌细胞;谷胱甘肽;Fe3O4;内吞作用摘要:目的在体外观察人肺腺癌细胞(SPC-A1)对谷胱甘肽(GSSG)修饰的纳米Fe3O4的内吞作用及与培养温度、时间及浓度的关系。方法通过透射电镜(TEM)与普鲁士兰染色法观察SPC-A1细胞对GSSG修饰的纳米Fe3O4的内吞作用。采用原子吸收光谱(AAS)法进行SPC-A1细胞粘附量及内吞量的定量分析。结果TEM结果表明,在4℃下培养24h后GSSG修饰的纳米Fe3O4吸附于SPC-A1细胞表面;37℃下培养1h后SPC-A1细胞可内吞GSSG修饰的纳米Fe3O4细胞数目明显增加。AAS分析表明,SPC-A1细胞表面纳米Fe3O4的粘附量同培养时间及浓度呈正相关。GSSG修饰的纳米Fe3O4浓度较低(01或04mg/ml)时,内吞量在24h后达到饱和;GSSG修饰的纳米Fe3O4浓度较高(07或10mg/ml)时,内吞量在72h后达到饱和,单个细胞内吞量最大可达160pg。结论SPC-A1细胞对GSSG修饰的纳米Fe3O4的内吞需要能量,内吞量依赖于浓度及培养时间。关键词:人肺腺癌细胞;谷胱甘肽;Fe3O4;内吞作用 ite(Fe3O4)nanoparticles YANZhubing,MAYongjie,GUHongchen,etal. InstituteofMicroandNanoScienceandTechnology,ShanghaiJiaotongUniversity(Shanghai200030,China) Abstract:ObjectiveTostudytherelationshipbetweenendocytosisofhumanlungadenocarcinomacelllineSPC-A1modifiedbyoxidizedGlutathione(GSSG)andcultureconditionsincludingculturetemperature,sobservedbytransmissionelectronmicroscopy(TEM)O4nanoparticlesweremeasuredbyatomabsorbspectrum(AAS).ResultsTEMresultsindicatedthatGSSG-modifiedFe3O4nanoparticlesadheredonthemembraneofSPC-A1cellafterbEingculturedat4℃for1hor24h,butSPC-Alcellcouldtookupnanoparticlesparticlesafterculturedat37℃fiedFe3O4nanoparticlesweremuchmaturatedafter24hwhenthenanoparticlesinlowconcentration(//ml),theuptakewassaturatedafter72hwhenthenanoparticlesinhighconcentration(//ml).time. Keywords:hu