青枯雷尔氏植物疫苗菌60 Co 诱变菌株的生物学特性.doc

文档介绍：青枯雷尔氏植物疫苗菌60Co诱变菌株的生物学特性
周游1,2,郑雪芳2,刘波2收稿日期:2012-10-22
基金项目:国家“863”计划项目(2012AA101504);国家星火项目(S2011C410006);福建省公益类科研院所基本科研专项(2011R1020-1);国家公益性行业农业科研专项(201303015)
作者简介:周游,女,硕士,主要从事茄科作物青枯病的生物防治研究。Tel:[phone],E-mail:
zhouyou.******@.
*通讯作者:刘波,男,1957年出生,福建泉州人,研究员,博士,主要从事微生物生物技术与农业生物药物研究。
通信地址:350003福建福州五四路247号福建省农科院,Tel:[phone],E -mail:fzliubo@
,黄建忠1,车建美2
(,福建福州 350008;,福建福州 350003)
摘要:利用60Co辐射诱变青枯雷尔氏菌无致病力菌株FJAT-1458构建其突变体库,比较诱变后菌株与出发菌株在生长特性、防效、定殖方面的异质性。结果表明,60Co 诱变处理菌株的最佳剂量为200Gy,%。52株60Co辐射诱变菌株中,有7株菌能提高对番茄青枯病的防治效果,为正突变,%; 有11株菌的生防效果明显低于出发菌株的防治效果,%,其中60Co 诱变后菌株FJAT-15022对番茄青枯病生防效果最好,在接种致病性青枯雷尔氏菌后第15d,%,明显高于出发菌株(%)。60Co 诱变后菌株FJAT-15022的生长特性与出发菌株FJAT-1458不同,菌株FJAT-15022能在较高温度(40℃)正常生长,而出发菌株FJAT-1458在此温度下不能生长;此外,二者的生长曲线不同,主要表现在对数生长期,在此期间,菌株FJAT-15022生长速率大于出发菌株FJAT-1458。60Co 诱变后菌株FJAT-15022的定殖特性也与出发菌株FJAT-1458不同,菌株FJAT-15022的定殖时间比出发菌株FJAT-1458延迟5d,但出发菌株FJAT-1458的定殖数量高于诱变后菌株FJAT-15022。本研究为研制防治青枯病的植物疫苗菌剂提供了基础资料。
关键字:青枯雷尔氏菌;诱变;温度;生物防效;定殖
The haracteristics of radioactive mutagenic Mutant of Ralstonia solanacearum by 60Co ray as a plant ine
Zhou You1,2, Zheng Xuefang2, Liu Bo2*, Huang Jianzhong1, Che Jianmei2
(1College of Life Science Fujian normal university, Fuzhou 350108, China;
2Biotechnology Institution, Fujian Academy of Agricultural Sciences, Fuzhou 350003)
Abstract: The present paper dealt with the biological characteristics of radioactive mutagenic of Ralstonia solanacearum by 60Co ray as a plant ine. In this study, tThe avirulent strain mutant library of Ralstonia solanacearum
(strain FJAT-1458) was treated constructed by 60Co ray to set up the mutant library, from which . Compared to the characteristics differences of characteristics, bio-control effefficiency ecton the bacterial wilt disease and , colonization on the host pared between the mutants and the original strain. The results showed that the optimum dose of 60Co mutagenic treatment was 200Gy., % of the