利用非经典分泌蛋白质的分泌信号实现脂肪酶A的分泌表达.doc

文档介绍：
利用非经典分泌蛋白质的分泌信号实现脂
肪酶 A 的分泌表达
崔静1,2,王光强2,陈海琴1,2,陈洁1,顾震南2,陈卫1,2,张灏2**
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(1. 江南大学食品科学与技术国家重点实验室,江苏无锡 214122;
2. 江南大学食品学院,江苏无锡 214122)
摘要:本文以枯草芽孢杆菌脂肪酶 A(LipaseA,即 EstA)为报告蛋白,尝试利用 4 种非经
典分泌蛋白质(non-classically secreted proteins)及其前 50 个氨基酸作为分泌信号以实现其分
泌表达。我们扩增了脂肪酶 A 的编码基因和非经典分泌蛋白质的编码序列,构建了 8 种针
对脂肪酶 A 的分泌表达载体,并转化枯草芽孢杆菌 WB800 菌株。结果表明部分非经典蛋白
质的编码基因或其前 50 个氨基酸序列能够引导脂肪酶 A 分泌至细胞外。
关键词:枯草芽孢杆菌;非经典分泌蛋白质;脂肪酶 A
中图分类号:Q786
Effect of non-classical secreted proteins on the secretion of
LipaseA
Cui Jing1,2, Wang Guangqiang2, Chen Haiqin1,2, Chen Jie1, Gu Zhennan2, Chen Wei1,2,
Zhang Hao2
(1. State Key of Food Science and Technology, JiangSu WuXi 214122;
2. School of Food Science and Technology,Jiangnan University, JiangSu WuXi 214122)
Abstract: In this study, we try to secrete the reporter protein lipaseA (estA) using the 4 selected
non-classical secreted proteins or their secretion signals. We amplied the coding sequence of the
secretion signal and the lipase A, constructed expression vectors for secretion of lipase A and
transformed these plasmids into Bacillus subtilis results showed that some
non-classical secretory proteins and their coding genes or the first 50 amino acids can direct the
secretion of the EstA.
Keywords: Bacillus subtilis; non-classical secreted proteins; LipaseA
0 引言
蛋白质分泌(protein secretion)是指蛋白质从合成的位置—细胞质经