文档介绍：
Comparative studies of paeoniflorin and albiflorin from
Chinese Paeony on Anti-inflammatory activities#
GAO Teng, WANG Qiangsong, CUI Yuanlu**
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(Research Center of Traditional Chinese Medicine,Tianjin University of Traditional Chinese
Medicine,Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of
Traditional Chinese Medicine, TianJin 300193)
Abstract: Purpose: Chinese Paeony has been used for more than 1000 years in traditional Chinese
medicine for the treatment of gynecological problems, cramp, pain, giddiness, and congestion.
Paeoniflorin and albiflorin, monoterpene glycosides isolated from Chinese Paeony, possess a variety of
pharmacological activities. The present study was investigated the anti-inflammatory activities of
paeoniflorin and albiflorin using models of harides (LPS) induced RAW cells.
Methods: Production of nitric oxide (NO) was measured by the Griess colorimetric method and
(ELISA). In addition, interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) synthesis were
analyzed using enzyme-linked immunosorbent assay (ELISA). The protein expression of
cyclooxygenase-2 (COX-2) was detected by cell-based ELISA. The gene expression levels of inducible
nitric oxide synthase (iNOS), COX-2, TNF-α, IL-6 were detected by quantitative real-time
reverse-transcription polymerase chain r