文档介绍：
iTRAQ 技术在植物中的应用#
薄乐涛,陈书燕,廖蕊,解美勤**
(兰州大学生命科学学院,兰州 730000)
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摘要:近些年来,随着生物科学技术的不断进步,蛋白质组学研究已经成为生命科学研究的
热点,其相关研究技术也如雨后春笋一般涌现,同位素相对标记和绝对定量(iTRAQ)技术
自从 2004 年被美国应用生物系统公司开发以来,依仗着自身独特的高敏感性和高准确性的
串联质谱及多维液相色谱联用,已经逐渐成为蛋白质组学定量分析的主流技术。iTRAQ 技
术可对复杂样本、细胞器、细胞裂解液等样本进行相对和绝对定量研究,具有较好的定量效
果及较高的重复性。由于其能够同时对最多 8 种样品进行标记分析,使得其在生命科学的各
个领域都取得了广泛的应用。在植物的蛋白组学研究中也取得了很大进展。本文对 iTRAQ
技术的原理、优缺点、实验流程以及在植物中的应用进展进行了综述。
关键词:植物学;同位素相对标记与绝对定量(iTRAQ)技术;蛋白质组学
中图分类号:Q-31
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Application of iTRAQ technology in plants
BO Letao, CHEN Shuyan, LIAO Rui, XIE Meiqin
(School of Life Sciences, Lanzhou University, LanZhou 730000)
Abstract: In recent years, with the radiply development of biological science and technology,
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proteomic research has e a hotspot in life science research, and its corresponding
methodological research has sprung up like mushrooms, in 2004, A new technique was developed
by Applied Biosystems : relative and absolute quantification(iTRAQ) technology, it has high
sensitivity and accuracy, particularly the bination of multi-dimensional liquid
chromatography and mass spectrometry (MS) based technologies with protein fragmentation
methods, it has gradually e the mainstream of quantitative analysis of proteomics
technology. The iTRAQ technology has a good quantitative effect and a high repeatability result
plex samples, organelles, and whole cell lysates.