利用表面等离子体共振研究TB-EPS在表面的吸附.doc

文档介绍：
利用表面等离子体共振研究 TB-EPS 在表面
的吸附#
张鹏,陈猷鹏,郭劲松**
5
10
(重庆大学城市建设与环境工程学院,重庆 400045)
摘要:紧密结合型胞外聚合物(TB-EPS)具有较强的絮凝和生物吸附能力,因而它们能修
复表面性质而促进细胞聚集。为了解 TB-EPS 在表面的吸附特性,精确测定 TB-EPS 在表面
上的吸附速率和亲和力,本研究从序批式生物膜反应器生物膜中提取 TB-EPS,采用表面等
离子体共振(SPR)来研究 TB-EPS 在金表面上的吸附情况。结果表明,TB-EPS 在金表面
吸附反应的结合速率常数(ka) = ± L/(g·s),解离速率常数(kd) = ± /s,
平衡结合常数(KA) = ± L/g,平衡解离常数(KD) = ± g/L。因此,
采用 SPR 能够方便快速地得到 TB-EPS 在表面的吸附动力学过程和吸附亲和力。
关键词:TB-EPS;吸附;SPR;表面
中图分类号:X 703
15
Investigation of TB-EPS Adsorption on Surface Using
Surface Plasmon Resonance
ZHANG Peng, CHEN Youpeng, GUO Jinsong
(College of Urban Construction and Environmental Engineering, Chongqing University,
20
25
30
35
40
ChongQing 400045)
Abstract: TB-EPS have strong ulation and biosorption abilities. Thus they can modify the
surface characteristics and promote cell aggregation. In order to understand the adsorption
characteristic of TB-EPS on surface, and determine the adsorption rate and affinity accurately, this
study investigated the adsorption behaviour of TB-EPS extraced from a sequencing batch biofilm
reactor using surface plasmon resonance (SPR). Results showed that the association rate constant
(ka) = ± L/(g·s) of the TB-EPS adsorption reaction on gold surface, the dissociation rate
constant (kd) = ± /s, the equilibrium binding constant (KA) = ± L/