文档介绍：
采用 SSR 和 SRAP 分子标记构建葡萄分子
遗传图谱#
郭印山,林洪,郭修武**
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(沈阳农业大学园艺学院,沈阳 110866)
摘要:以葡萄品种‘87-1’为母本,优良品系‘9-22’为父本,杂交创建了 F1 群体。从该
群体中随机选用 149 个单株组成作图群体,采用 SSR 和 SRAP 分子标记分析,并运用
软件进行连锁分析,分别构建了母本‘87-1’和父本‘9-22’的分子遗传图谱以
及双亲的共有图谱。其中,‘87-1’的遗传图谱包括 21 个连锁群,包含 163 个标记位点,
覆盖总图距 ,位点间平均遗传距离为 ;‘9-22’的遗传图谱包括 20 个连锁
群,含有 158 个标记位点,覆盖总图距 cM,位点间平均遗传距离为。共有图
谱包括 20 个连锁群和一个二连体,包含 217 个标记,图谱总长为 ,标记间平均距
离为 ,连锁群的平均长度为 cM。图谱覆盖了葡萄基因组的 19 条染色体,该图谱
的构建为今后进一步开展相关性状的 QTL 定位和分子标记辅助选择等研究奠定了基础。
关键词:欧亚种葡萄;遗传图谱;SSR;SRAP
中图分类号:
SSR AND SRAP MARKER BASED LINKAGE MAP OF
Vitis vinifera L.
GUO Yinshan, LIN Hong, GUO Xiuwu
(College of Horticulture, Shenyang Agricultural University,shenyang 110866)
Abstract: An F1 population was created by the cross ‘87-1’×‘9-22’. The female parent 87-1 was
an extremely early maturing cultivar with strong flavor. The male parent was an excellent
breeding line producing large berries maturing late. 149 individuals were randomly chosen as the
mapping population. Molecular ic map for each parent as well as the consensus map were
constructed using simple sequence repeat(SSR)and Sequence-Related
Amplified
Polymorphism(SRAP)marker by software JoinMap . The 87-1 map covers a total length of
cM distributed in 21 linkage groups, and consists of 163 molecular markers with an
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