GANT61联合Cyclopamine对胃癌细胞增殖和凋亡作用研究.doc

文档介绍：
GANT61 联合 Cyclopamine对胃癌细胞增殖
和凋亡作用研究#
郭绪峰,董卫国,王军,余世界,宋佳,刘敏**
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(武汉大学人民医院消化内科,消化系统疾病湖北省重点实验室,武汉 430060)
摘要:目的:探讨 Hedgehog(Hh)信号通路抑制剂对人胃癌 BGC823 细胞增殖与凋亡作用研
究。方法:Cyclopamine(环巴明)特异性阻断 Hh 信号通路后,联合应用 Gli-1 特异性阻滞
剂 GANT61,采用 MTT 法检测细胞存活率,Annexin V-FITC/PI 双染流式细胞术检测其对细
胞凋亡的影响,Hochest33258 观察凋亡细胞核形态学改变。结果:MTT 检测 GANT61 和
Cyclopamine 的半数抑制浓度分别为 , 。联合应用 GANT61 后,流式细
胞术检测 BGC823 细胞的凋亡率明显增加。Hochest 实验结果显示,联合给药组凋亡细胞核
比例较单独给药组高(p<)。结论:阻断 Hh 信号通路能显著抑制 BGC823 细胞的增殖,
促进细胞凋亡。GANT61 联合应用 Cyclopamine 可加强胃癌细胞的凋亡,为靶向治疗胃癌提
供一种新的治疗思路与决策。
关键词:胃癌;GANT61;Cyclopamine;Hedgehog 信号通路;增殖;凋亡
中图分类号:
Effect of bined with Cyclopamine on the
proliferation and apoptosis of BGC- 823 Cells
Guo Xufeng, Dong Weiguo, Wang Jun, Yu Shijie, Song Jia, Liu Min
(Department of Gastroenterology, Renmin Hospital of Wuhan University, Key Laboratory of
Hubei Province for digestive System Disease, WuHan 430060)
Abstract: Objective: To explore the effect of Hedgehog(Hh) pathway inhibitor on proliferation
and apoptosis of BGC823 cells. Methods: The oinhibition rate of BGC823 cells was detected by
MTT assay and flow cytometry after exposed to GANT61 and Cyclopamine. The assay of
Hochest 33258 was used to detect the apoptotic nucleus. Results: The IC50 of GANT61 and
Cyclopamine detected by MTT is and . The early apoptosis of BGC8