文档介绍：宁波地区汉族人群冠心病候选基因变异及其临床的关联性【摘要】目的:探讨CETPTaqlB,:入选宁波地区行冠状动脉造影(CAG)检查的汉族人群186例,%,平均年龄(61±10),***仿异丙醇抽提外周血白细胞全基因组DNA,通过聚合酶链式反应(PCR)及限制性片段长度多态性(RFLP)结合测序方法确定候选基因ABCA1G596A单核昔酸多态性(SNPs)・结果:宁波地区汉族人群CETPTaqlB基因型B1B1,,;LPL Hindlll基因型H+H+,H+H-及H-H-,;LPLPvuII基因型P+P+,P+P-及P-P-,(),同时对TG亦有降低趋势(P=);LPLHindlll及PvuII对HDLC均无显著影响,而LPLHindlll及PvuII基因变异均显著降低血浆T***平(,);(P>).结论:冠心病候选基因CETPTaqlB,LPLHindlll及LPLPvuII变异对血脂代谢产生一定的影响,但与冠心病临床表型Z间缺乏显著性关联.【关键词】冠状动脉疾病;多态性,单核背酸;脂类;胆固醇酯转运蛋白;脂蛋白脂酶[Abstract]AIM:ToinvestigatetheeffectsofvarcationofCETPTaqlB,:Totally186patientsundertakingcoronaryangiographywererecruitedfromNingbodistrict,including111pationlswithcoronaryarterydisease(CAD)and75controls・%andaged(61±10)years・TheDNAwasextractedfromperipheralwhitebloodcellsbyproteinaseKdigestion,(SNPs)ofcholesterolestertransferprotein(CETP)TaqlB,LipoproteinLipase(LPL)HindlllandLPLPvuIIweregenotypedbyPCRRFLP,andverifiedbygenesequencing・RESULTS:GenotypefrequenciesofCETPTaqlB,,,B1B2andB2B2,,+H+,H+H-andH-H-,&+P+,P+P-andP-P-,(P=),wh订eTGlevelhadatrendofdecrease().SNPsofLPLHindlllandLPLPvuIIpredictedplasmaTGlevel().,CETPTaqlB,LPLHindlllandLPLPvuIIvariantswerenotpredictiveofthepresenceofcoronaryangiographicdefinedcoronaryarterydiseaseinNingbodistrietHanpopulation(P>