漆酶催化麻织物固定化溶菌酶及其抗菌性能.doc

文档介绍：
麻织物的漆酶催化溶菌酶固定化及其抗菌
性能研究#
董爱学1,王强1,范雪荣1,王平1,崔莉1,袁久刚1,章金芳2**
5
10
15
20
25
30
(1. 江南大学纺织服装学院,无锡 214122;
2. 新天龙集团有限公司,上虞 312369)
摘要:用漆酶对木质素的催化作用将溶菌酶接枝到富含木质素的麻织物上,赋予其抗菌性。
通过考马斯亮蓝染色法证明了溶菌酶的接枝,对两种不同木质素含量的织物(棉/亚麻混纺
织物、黄麻织物)的接枝效果进行了比较,并应用扫描电镜(SEM)考察了接枝后麻纤维表
面形态,研究了溶菌酶固定化后麻织物的抗菌性能和耐洗稳定性。结果表明:麻织物经漆酶
催化可实现溶菌酶的固定化,麻纤维木质素含量越高,溶菌酶接枝越多,整理后棉/亚麻织
物和黄麻织物抑菌率分别达到 %和 %,且通过接枝法固定溶菌酶的麻织物较吸附
固定法具有更好的耐水洗稳定性和抗菌效果。
关键词:溶菌酶;固定化;漆酶;抗菌;麻织物
中图分类号:TS195
Immobilization of Lysozyme Catalyzed by ase on Bast
Fabrics and its Antibacterial Property
DONG Aixue1, WANG Qiang1, FAN Xuerong1, WANG Ping1, CUI Li1, YUAN
Jiugang1, ZHANG Jinfang2
(1. College of Textiles and Clothing, Jiangnan University, Wuxi 214122;
2. Xin TianLong Group Co., Ltd., Shangyu, 312369)
Abstract: Grafting of lysozyme catalyzed by ase on bast fabrics was studied. The antibacterial
properties of fabrics were obtained. The graft of lysozyme was proved by method of dyeing with
coomassie brilliant blue. The grafting effects of two kinds of fabrics with different lignin content
(cotton/flax fabric and jute fabric) pared. Scanning electron microscopy (SEM)
presented the surface morphologies of grafted bast fibers. The washing stability and the
antibacterial property of lysozyme immobilized bast fabrics were determined. The results showed
that lysozyme can be immobilized on bast fabrics via the catalysis of