文档介绍：
无菌松材线虫的制备方法#
赵海娟,刘倩**
(中国农业大学植物病理系,北京 100193)
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摘要:松材线虫病是导致松树迅速死亡的一种毁灭性病害。目前松材线虫病的致病机理尚未
研究清楚,对于松材线虫是唯一病原还是由松材线虫和细菌共同引起的仍存在争议,而获得
无菌松材线虫是解决这一争议的首要条件。本文采用了多步消毒方法,除了对松材线虫进行
多步消毒外,由消毒松材线虫产生的虫卵也同样经过多步消毒处理,然后采用细菌通用引物
结合 PCR 检测消毒效果,获得了真正无菌的松材线虫。同时,本研究发现本文选用的消毒
剂对松材线虫虫卵孵化没有显著影响,且无菌松材线虫的繁殖量显著高于带菌松材线虫。
关键词:植物病理;松材线虫;消毒
中图分类号:
A method of obtaining sterile pine wood nematodes
ZHAO Haijuan, LIU Qian
(Department of Plant Pathology, China Agricultural University, Beijing 100193)
Abstract: Pine wilt disease caused by pine wood nematode is one of the most devastating forest
diseases in the world. The pathogenicity mechanism of Bursaphelenchus xylophilus have not been
well understood. Recently, a series of studies have shown that bacteria may play an important role
in pathogenicity of the disease. However, this hypothesis is still controversial. To assess the role
of bacteria in pine wilt disease, sterile B. xylophilus was obtained by multiple sterilization steps
combined with the method of PCR-based detection of bacterial contamination. Reproduction of
germy and germfree B. xylophilus pared.
Keywords: Plant Pathology; Bursaphelenchus xylophilus; sterilization
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0 引言
松材线虫病是松材线虫(Bursaphelenchus xylophilus) 引起的松树的一种毁灭性病害,
又称松树萎蔫病。松材线虫病具有导致树木死亡速度快、传播蔓延迅速、防治难度大等特点,
松树一旦感染松材线虫,最快的 40 天左右即可枯死,幼苗感病后 20 多天即可枯死,3-5 年
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便可造成大面积毁林[1]。我国于 1982