文档介绍：Study on Immobilized Glucose Oxidase with Supports of Crosslinked Chitosan-diamineA Thesis Submitted to Chongqing University in Partial Fulfillment of the Requirement for theMaster’sDegree of ScienceByYingli WangSupervised by prof. Xiaohua ZhouSpecialty:Chemical Engineering & TechnologyCollege of Chemistry and Chemical Engineeringof Chongqing University, Chongqing, ChinaOctober, 2012重庆大学硕士学位论文中文摘要I摘要固定化酶技术经过半个多世纪的发展后,柔性固定化,即在固定化载体上接上一些足够长度的亲水分子链(柔性链),再将酶键合到柔性链上的固定化技术,成为该技术领域的研究热点。该柔性链可提高载体表面的柔韧性、亲水性,减少固定化过程中酶分子所受到的碰撞力,从而较好的维持酶蛋白的正常构象和自由度、保留其游离态的均相催化活性。本研究以乙二胺、己二胺、癸二胺为柔性臂,制得交联壳聚糖-二胺衍生物微球;并以此微球为载体,对葡萄糖氧化酶(GOD)进行固定化。研究表明,乙二胺、己二胺、癸二胺-交联壳聚糖(ethylenediamine-,hexamethylenediamine-,decamethylenediamine-crosslinked chitosan,D)为载体时,,所需酶/载体氨基摩尔比、戊二醛浓度、交联时间各不相同。H所需的酶/载体氨基摩尔比和戊二醛浓度最大,×10-4、;交联时间最短,。对最佳条件下制备的固定化GOD进行荧光分析,H微球的内表面积最大、孔道分布最均匀,因此固定化酶活力回收最高。固定化GOD的动力学研究表明,扩散系数(De)、载体半径(r0)、底物浓度(s0)对于固定化酶的动力学有不同程度的影响。扩散系数与载体半径的影响较显著;且扩散系数越大,载体半径越小,越有利于底物扩散。底物浓度对于固定化酶的动力学的影响较小,但适当提高底物的浓度可提高反应速率、节约生产成本。三种柔性手臂接枝的固定化酶的耐酸性、耐热性优良,重复使用稳定性好,可长久储存。4℃H为载体时,%;,CCD为载体时,、、%。关键词:壳聚糖,二胺,固定化,动力学重庆大学硕士学位论文英文摘要IIABSTRACTFlexible immobilization has interested many researchers these years since immobilization was developed fifty years ago. Supports are grafted with hydrophilic chain, the flexible chain, where enzymes are immobilized and flexible immobilization is conducted. The flexible chain makes it more flexible and affinitive, and collision force of enzymes is declined. Thus the technology benefits enzymes by remaining natural conformation, degree of freedom and homogeneous catalytic , hexamethylenediamine and decamethylenediamine were grafted to the crosslinked chitosan microsphere. Then the glucose oxidase(GOD) was immobilized on each of into account the effects of inner diffusional limitation (effectiveness factors, De), carrier size (r0) andsubstrate