文档介绍：TouchdownPCR扩增多样性小鼠Fab抗体基因扩增多扩性小鼠抗体TouchdownPCRFab基因中震扩通国搪2010,26(23):22-25ChineseAgriculturalScienceBulletin扩增多扩性小鼠抗基因体TouchdownPCRFab王乃扩何浪薛立群,,湖南扩扩大扩物院学医学扩沙(,410128)摘要扩了扩增多扩性抗基因扩物体保扩菌抗扩的足扩扩容噬体体分离鼠:Fab,.C57BL/6的脾扩并,制扩脾扩胞扩液提取其扩逆扩扩扩利用普通和扩,RNA,cDNA,PCRTouchdown(TD)PCR增鼠基Fab因扩物通扩扩脂糖凝扩泳扩扩胶扩物比扩分析扩方法的扩增效果扩果扩示扩扩和重扩,PCR,2.:Fd基因的扩物大小扩扩与理扩扩相符扩泳扩果扩示扩得个重扩基PCR680bp,.,TD—PCR8Fd因和个6扩扩基因扩条普通扩得个重扩基因和个条扩扩基因扩在普通扩物中扩扩基,,因引物未能扩增出相扩基因而扩增出扩基因扩增出引物和vB,TD——PCRIliA的扩扩基因IIIC,而普通却未扩扩扩扩果普通扩增出引物的扩扩基因未扩扩扩扩果PCR,PCRIIIB,TD—,TD—PCR,Fab,:;;中扩分扩号文扩志扩献扩文扩号:Q782:A:,HeLang,XueLiqun(Collegeofveterinarymedicine,HunanAgricultureUniversity,Changsha410128)Abstract:ToamplifyvariousproductsofFabantibodygeneswithdiversity,,spleencellsuspensionwasprepared,totalRNAwasextracted,,theproductswereanalyzedbyagarosegelelectrophoresis,,andtheresultco~-PCRhad8highchaingeneand6lightchaingene,,andappearedintheproductsofTD—-PCR,,andnotappearedintheproductsofTD-—PCRmayincreasediversityofFabantibodygenes,isanapproachtoensurereservoircapacityofphageantibodylibrary,:mouse;phagedisplaytechnology;antibodylibrary基金扩目国学家自然科基金扩用鼠源性高扩和力菌噬体抗扩扩和克隆新的血体:"Fab清学抗原基因湖南省育扩科教学H—Y"(30901090/C120209);研青究扩秀年扩目利月高扩和力菌抗扩扩血