文档介绍：Two-TemperatureHybridizationforMicroarrayDetectionofLabel-FreeMicroRNAswithAttomoleDetectionandSuperiorSpecificity —在两个温度下杂交进行miRNA微阵列检测一种高灵敏度高特异性的的方法JeongMinLeeandYongwonJung*褒错危红蛋绍闰训盯蝎路膜汇岩襄否儡航桐吸砌筒嘿路夫身煽描猫磨峰扒miRNA微阵列检测miRNA微阵列检测smallsizeandlowabundanceofmiRNAs—improvingthedesignofprobesandthelabelingofthemiRNAs→)Thesecaptureanddetectionprobeswere10-12ntinlengthwithdifferentnumbersofG,C,,whicheachrangedfrom25℃to58℃.)FluorescenceimagesofmicroarraydetectionofhumanLet-7a(top)andmiR96(bottom),andthecorrespondingmiRNAswithasinglebasemismatch(Let-7candmiR96-C,sequencesinFigure2a)undervarioustwo--temperaturearraydetectionofmiRNAswithtwoLNAprob