文档介绍：.(A)SchematicrepresentationofPancreaticDuctalAdenocarcinomaAssociatedNeuralRemodeling.(A,Left)NeuralRemodelingwithinfiltrationofnervefiberswithinPancreaticDuctalAdenocarcinoma.(Right)PerineuralInvasionrepresentingtheinfiltrationoftumoralcellswithinnervefibers.(B)partmenttranscriptomicsignaturefromHumanPDAsamples(n=4).(C)(RNAintegrityNumber)wasmeasuredbyAgilent2100bioanalyzer.(D)Heatmaphighlightingtranscriptsmarkedas“AxonGuidance”foundtobesignificantlyover-(S)ortumor(T)..“Axonguidance”familyexpressioninvitro.(A)posingthe“Axonguidance”familyanalyzedbyQRT-PCRinmousepancreatictumoralcellline(PK4A,usedasnormalizer),mousemacrophages(M),P<;darkgreen,P<(n=3).-tumoralmicroenvironmentinduceschangesinprimarysensoryneuronsandSchwanncellbehaviors.(A)Representativeimages(allat20Xmagnification,scalebar:100μm)worksimmunofluorescentlylabeledwithβ-tubulinasneuronalmarker.(B)HumanSchwanncellproliferationwasmeasuredbyMTSassayafter2