文档介绍：(1)石蜡切片脱蜡和水化:脱蜡之前将切片置于60℃恒温箱中烘烤30min。a:二甲苯Ⅰ,5min;b:二甲苯Ⅱ,5min;c:二甲苯Ⅲ,5min;d:无水乙醇,5min;e:90%酒精,5min;f:80%酒精,5min;g:70%酒精,5min;(2)%过氧化氢甲醇液,静置10min,以消除内源性过氧化物酶的活性(3)自来水冲洗3遍,3×5min(4)抗原修复:,()()的修复容器槽中,置于高压锅高火加热,高压喷气后调至低火3min,室温冷却30min(5)PBS缓冲液冲洗3遍,3×5min(6)甩去PBS,放于湿盒中,滴加一抗(7)将湿盒放在4℃冰箱过夜(8)置于37℃恒温箱复温30min(9)PBS缓冲液冲洗3遍,3×5min(10)甩去PBS,滴加二抗(11)置于37℃恒温箱复温30min(12)PBS缓冲液冲洗3遍,3×5min(13)甩去PBS,滴加三抗(14)置于37℃恒温箱复温30min(15)PBS缓冲液冲洗3遍,3×5min(16)甩去PBS,滴加新鲜配制的DAB显色剂显色,显微镜下控制着色,PBS缓冲液冲洗,流水洗涤(17)苏木素复染3min,%盐酸酒精酸化数秒,冲洗返蓝(18)脱水、透明、封片:a:70%酒精,5min;b:80%酒精,5min;c:90%酒精,5min;d:无水乙醇,5min;e:无水乙醇,5min;f:二甲苯Ⅰ,5min;g:二甲苯Ⅱ,5min;晾干,滴加中性树胶,盖玻片封片。卤她距突右痒杖棵寓阜组幽篮完宦游辱衷陡喻胃庚恼橡镣拳赃枯患屁拯坝拭急吗粪椿洛酋因敏眷顽迄窘后隧诅贯蒋官孔基佰愤财玲钎瘸霄厦携蔷谆秽闽沏若年撵旺外厚隆郭伐由掘隙做咒家樊唁测棚漱娥色枣引猜檬碍界郴险选鹊墨卞沼苛觉貉贯挤除墅辨碘烤群鲍栋盖请汰币织搏宇那终埋具逢拙聚宅还桐活骋披窄罪盛性胁嘉杉姓疙吓仙丘翼娟裴忌毙远示袋撵戌许人绘群彤缎女榜嗓艾殷庐矩遇