文档介绍：Certificate of Analysis
Cat. No.
Part No. Pfu DNA Polymerase
Lot No.
Order Date Storage condition: The undiluted enzyme solution is stable when stored
at -20℃.
Activity:2U/ul
Storage and dilution buffer: 20mM Tris-HCl; 1mM dithiothreitol;
EDTA; KCl; Nonidet P40, %(v/v); Tween 20,
All ShineGene Inc.
Products are assayed %(v/v); glycerol, 50%(v/v); pH (4℃).
as needed to ensure Unit definition: One unit is defined as the amount of enzyme required to
performance at the
given specifications. catalyze the incorporation of 10mmols of dNTP into an acid-insoluble
ShineGene Inc. material in 30 minutes at 74℃. The reaction conditions are: 50mM
certifies this product
will perform to the Tris-HCl, (pH at 25℃), 50mM NaCl, 10mM MgCl2, 200uM dATP,
stated specification is dCTP, dGTP and radiolabelled dTTP, and activated calf thymus
handled properly.
DNA in a 50ul reaction.
10╳Reaction buffer : (200mM Tris-HCl (pH at 25°C), 100mM KCl,
100mM (NH4)2SO4, 20mM MgSO4, % Triton X-100 and 1mg/ml
Nuclease-Free BSA)
Note: It is mended to add dNTPs to this incubation mixture shortly
before use. This is to prevent position of the deoxynucleoside
thiphosphate that occurs during Prolonged storage at the alkaline pH
values required for optimal enzyme activity.
Quality cont