文档介绍：FAQ (Frequently Asked Questions)
about custom DNA Synthesis
1. What is scale of synthesis?
Scale of synthesis refers to the amount of starting CPG (controlled-pore glass) support-bound monomer used to
initiate the DNA synthesis, not the amount of final material synthesized. As an example, a 20 mer synthesized
at a 200 nanomole scale of synthesis will produce approximately 80 nanomoles. The losses occur during
synthesis, post-synthetic processing, transfer of material, and quality control.
2. Do I need to have my oligo purified?
It depends on whether or not modifications are requested and what the application will be. Failure sequences
may be generated both during the synthesis and post-synthesis processing. We mend that all
modifications be purified either by cartridge or HPLC. For mended purity and scale (based upon
application), please see Table I.
Table I
mended Scales of Synthesis and Purification
Application Scale of Synthesis Purification
For Non-modified Oligos

DNA sequencing 50 or 200 umol OPC OR PAGE
PCR (general mplification) 50 or 200 umol OPC
PCR (diagnosticapplication) 200 umol OPC OR PAGE
Subcloning, site-directed mutageneses or cDNA synthesis 200 umol OPC\PAGE\HPLC

Gene Construction
<80mer 200 umol PAGE
>80mer umol PAGE
Antisense um or more PAGE\HPLC
For Modified Oligos

Modified bases and chemical linkers 200 umol PAGE\HPLC
Reporter groups (biotin, DIG or fluorescent dyes) 200 umol PAGE\HPLC
3. How much do I get or what scale of synthesis should I order?
Refer to the Table II
1
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Table II
Scale of Synthesis, Purification and Yields
Scale of synthesis Purification Yield

150 ug: actual yield is sequence and
50 nanomole Desalt
length dependent
Reverse phase cartridge Average yield: 50-75 ug
Reverse phase HPLC Average yield: 50-75 ug
Anion-exchange HPLC Average yield: 50-75 ug

200 nanomole Desalt Avera