文档介绍：浙江大学硕士学位论文从酵母细胞中分离纯化S-腺苷-L-蛋氨酸和谷胱甘肽的研究姓名:游建锋申请学位级别:硕士专业:生物化工指导教师:林建平 20040301 浙江大学硕士学位论文摘要 S一腺苷L一蟹氨酸(S-adenosyl L—methionine,SAM e或AdoMet,SAM)和谷胱l二|肽(Glutathione,GSH)是生物体内普遍存在的两种重要的生物活性小分子,本文主要对从酵母细胞中分离纯化这两种物质的工艺以及SAM稳定性黼的制各进行了研究。首先,。肽,对谷胱甘肽在HZ001树脂离子交换柱I二的动态离子交换行为进行了研究,优化了上柱和洗脱条件,使得本步离子交换谷胱甘肽的回收率达到了9l%。然后,筛选了一种高选择性、高吸附容量的弱酸型阳离子交换树脂JKll0, 考察了SAM在JKll0树脂上的离子交换动力学,确定了相关的参数,研究了SAM 在JKllO树脂上的静态离子交换平衡行为,拟合了吸MJ等温线。还对SAM在 JKll0树脂柱上的动态离子交换进行了研究,确立了优化的工艺条件,使得JKll0 树脂对SAM的吸附容量达到172mg/g二F树脂,本步离子交换SAM回收率93%, SAM纯度95%。并且由于谷胱甘肽在JKll0树脂上没有吸附,上柱流出液可以重新进入谷胱甘肽的分离工艺。最后还对高稳定性的SAM硫酸对甲苯磺酸双盐的制备进行了研究,获得了纯度达98%的SAM硫酸划甲苯磺酸双盐。本论文开发了一条从酵母细胞中同时分离提纯SAM和GSH的工艺路线, 为SAM和谷胱甘肽的工业化生产奠定了良好的基础。关键词:SAM 谷胱甘肽酵母分离离子交换树脂离子交换动力学离子交换平衡模型稳定性盐浙江戈学硕士学位论文 Abstract S-adenosyl—L-methionine(SAM·e,AdoMet or SAM)and g[utathione(GSH)were two important molecules widely existed in various cells The separation and purification ofthem as well asthepreparation ofstable SAM salt were studied acidanion ion-exchange resinHZ00 1 was chosen toisolateGSH from the extract ofspent yeast cell,and itscapacity forGSH was 8,lm∥ml(wet resin)The separation process ofSAM was performed in afixed—bedion-exchange column,and theoperation conditions were optimized for theseparation ofGSH from recovery ofGSH reached 91%. Secondly,the ion·exchange separation process ofSAM from the extract ofyeast cell was theresinsused,JKl 10 resinwas thebest one and itscapacity forSAM was (dry resin)while GSH was hardly adsorbed SoGSH could beseparated and purified by HZ001 resinconsequently The ion-exchange velocity and dynamic column process ofSAM on JKll0 resin were equilibrium isotherms were obtained atvarious pH values andtemperatures andtherelatedequilibrium models were examined The ion-exchange ics ofSAM+/H—system based on JKI10resin was alsodiscussed And the rate。limitingstep of theion-exchange pr