文档介绍：青岛大学硕士学位论文咪达唑仑对大鼠杏仁核点燃抑制作用的研究姓名:宋力申请学位级别:硕士专业:药理学指导教师:岳旺 20050315 中文摘要 Y909033 咪达唑仑对大鼠杏仁核点燃抑制作用的研究硕士研究生:宋力导师:岳旺教授摘要咪达唑仑是一种新型苯二氮卓类药物,由于其消除半衰期短,顺行性遗忘和安全性高,同时具有较强的镇静,抗惊厥和抗焦虑等优点,已广泛应用于临床麻醉中。国内外大量实验已经证明,癫痫发作与脑内抑制性氨基酸递质1一氨基T酸(GABA)和兴奋性递质谷氨酸的平衡失调有关川,也就是说,脑内兴奋性氨基酸和抑制性氨基酸二者含量一旦失去平衡,就会导致癫痫的发生口1“。本文通过应用咪达唑仑于大鼠杏仁核电刺激点燃模型,研究咪达唑仑的抗癫痫作用,为临床开发新的药用价值提供实验依据。目的:本文旨在制备大鼠杏仁核点燃模型,研究眯达唑仑对大鼠杏仁核点燃发作的抑制及其抗癫痫作用。方法:建立大鼠杏仁核点燃模型,测定不同剂量的咪达唑仑对点燃模型各项指标的影响,探讨眯达唑仑的抗癫痫作用;观察眯达唑仑对小鼠自主活动的影响;利用小鼠最大电休克惊厥模型计数惊厥发生率。结果:咪达唑仑(—·kg~,ip)可剂量依赖性抑制杏仁核点燃发作,缩短后放电时程(ADD),降低Racine’s分级(尸<);咪达唑仑(— ~,ip)能明显降低小鼠自主活动数(.P<),同时可以剂量依赖性地对抗小鼠最大电休克发作,降低惊厥次数和惊厥发生率(P<)。结论:咪达唑仑具有抑制杏仁核点燃的作用,抗癫痫作用显著,具有明确的抗惊厥和镇静作用。该项研究为临床合理用药,开发咪达唑仑新的药用价值提供理论和实验依据。关键词:咪达唑仑;杏仁核点燃;癫痫;最大电休克;自主活动英文摘要 STUDY 0F INHIBITION oF MIDAZOLAM oN AMYGDALAⅪNDLING INRATS Abstract 】Ⅵidazolam is a new—type ofBenzodiazepirles nledicine,it has been widely adopted inclinicalanestllesiabecause ofitsadvantages,such asshort preclud half-life,ante】?ogmcle a11111esiaand high it possess omer vinues:strong seda畦on,anticonvulsion and scudy was des噜ned to useamy鲥ala kindling model inmts,toinvestigate me antiepileptic r01e of rnidazolam,and supply exp嘶ment fundation for reasOnable medication inclinical. Aim To inVestigate the inhibition and ajltiepil印tic effect of nlidazolam onamygdala kindling models in rats. Methods Toestabhsh amygdala kindling models in ratsaIlddeteⅡnine the ef!fectof different dose ofmidazolam on l(indling models and its antiepiI印tic effect;to observe tllee虢ct ofIIlidaz01am on independence actiVityof血ce;convulsions weredete删ned by n】aximalel elecⅡDshock seizure(MES)modelin面ce. Results Midazolam(—·kg~,ip) were shown to dose— dependenny inhibitamygdala kindled seizure inmts(P<),significantly shorten the afterdischa曙duration(ADD)and reduce me Racine’s stage (P<);Midazolam(—-砖~,ip)can signi&anny decrease the independence activity number inmice(P<).Meanwhile,IIlidazolam c