文档介绍:;;.自发性荧光物质神经细胞和心肌细胞等内的脂褐素呈棕黄色荧光。肝贮脂细胞和视网膜色素上皮细胞内的维生素A呈绿色荧光。某些神经内分泌细胞和神经纤维内的单***类物质(儿茶酚***、5-羟色***、组***等)在甲醛作用下呈不同颜色的荧光。组织内含有的奎宁、四环素等药物也呈现一定的荧光。嗜中性粒细胞中脱氢酶辅酶呈现一定的荧光。..优点:检出能力高对细胞的刺激小能进行多重染色用途:物体构造的观察荧光的有无、色调比较进行物质判别发荧光量的测定对物质定性、(阻挡滤片).-styleframearecapableofproducingfluorescenceilluminationeitherthroughepiscopicordiascopicopticalpathways,-(diascopicilluminator,condenser,fielddiaphragm,filters,etc.)arebuiltintothemainframe,"cubes"thatcontainamixtureofinterferencefiltersincludingabarrierfilter,dichroicmirror,,lightemittedfromthelamppositionedintheepiscopiclamphousepassesthroughacollectorlensandthenthefieldandaperturediaphragmsbeforeenteringthefirstinterferencefilterinthecubeset,,emittedbyfluorophoresresidinginthespecimen,travelsbackthroughtheobjectiveandthedichroicmirrorbeforepassingthroughabarrierfilterandintothemicroscopeeyepiecesorcamerasystem..激发光源高压***灯拥有313,337,365,405,436,546,and577纳米的峰值谱。高压Xenon灯有连续的可见光光谱范围,光源强度比较平均。***灯光谱.