文档介绍：常见蛋白质标签总结2008-12-0822:06一、氨基酸标签(含小肽标签)(Histag)—般为6个组氨酸,用Ni2+(Cu2+)亲和层析纯化FLAGtag:N-DYKDDK-C,recoveredwithspecificantibodyHAtag:anepitopederivedfromtheInfluenzaproteinhaemagglutin(HA,禽流感病毒血凝素),-YPYDVP-,CrecoverywithanHAantibodyMYCtag:anepitopederivedfromthehumanproto-oncoproteinMYC,-ILKKATAYIL-C,N-EQKLISEEDL,-CrecoverywithanMYCantibodySBPtag:StreptavidinBindingPeptide,链霉亲合素结合肽,38aminoacidtag(MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQ更多参考在SigmaCBPtag:钙调蛋白结合肽(CBP;26aa钙调蛋白结合肽与钙调素结合是Ca2+依赖的,这种结合不受标签所处的位置影响(N端和C端均可),在中性pH条件下使用2mMEGTA可以很方便的将目标蛋白洗脱下来。这一系统有如下优点: 1特异性很高,因为大肠杆菌没有可以和钙调素结合的蛋白; 2与His标签相比可以在强还原性条件下纯化。纤维素结合肽(CBD):能与纤维素介质特异性的结合,可以在温和的条件下洗脱(乙二醇或低盐条件),pETCBD载体含有纤维素结合肽(CBD的序列,可方便构建。二、,:thesmallglutathione-S-transferase(GST;26kDa,)recoverybyaffinitytosubstrateglutathioneboundtoacolumn,:麦芽糖结合蛋白(MBP;40kDa载体:pMALIMPACT:Intein-MediatedPurificationwithanaffinityChitin-bindingTag(几丁质结合肽)硫氧还蛋白:hiol-:a40-,mostnotablyIgG':,:绿色荧光蛋白(GFP;三、化学标签biotin-streptavidin(生物素-链霉亲合素)linkingaminoacids,oftenlysines,tocofactorandvitaminbiotinpurificationbytightbindingtostreptavidin-agaroseor-,anditlacksavidin'(pI)of~(proteintag)是指