文档介绍：Image analysis for biology MBL Physiology Course 2008 Thanks to Hao Yuan Kueh, Eugenio Marco, Mike Springer and Sivaraj Sivaramakrishnan 1) Why Image Analysis?................................................................................................. 2 Image Analysis Strategies............................................................................................... 2 Layout of this tutorial...................................................................................................... 4 2) Basics...................................................................................................................... .... 5 Image types, data classes and image classes................................................................... 7 Basic Segmentation using Thresholding......................................................................... 9 Image histograms........................................................................................................ 9 Metamorph Stack Files ............................................................................................. 13 EXERCISES................................................................................................................. 14 3) Contrast adjustments................................................................................................. 15 4) Spatial Filtering......................................................................................................... 18 Smoothing filters........................................................................................................... 20 Edge detection filters .................................................................................................... 21 Laplacian filter.............................................................................................................. 2 2 Median filter........................................................................................