文档介绍：Illumina测序技术概述Part#?2013Illumina,,IlluminaDx,BaseSpace,BeadArray,BeadXpress,cBot,CSPro,DASL,DesignStudio,Eco,GAIIx,icEnergy,GenomeAnalyzer,GenomeStudio,GoldenGate,HiScan,HiSeq,Infinium,iSelect,MiSeq,Nextera,NuPCR,SeqMonitor,Solexa,TruSeq,TruSight,VeraCode,thepumpkinorangecolor,icEnergystreamingbasesdesignaretrademarksorregisteredtrademarksofIllumina,:测序流程中的主要步骤清楚了解Illumina–)的过程ClusterGeneration–能够描述簇生成(SequencingbySynthesis)的原理理解边合成边测序(–FORRESEARCHUSEONLYPart#-RapidHiSeqSequencingHiScanSQ测序GAIIxMiSeqNextSeqICS/RTADataAnalysisCASAVA数据分析MSRBaseSpaceFORRESEARCHUSEONLYPart#-RapidHiSeqSequencingHiScanSQ测序GAIIxMiSeqNextSeqICS/RTADataAnalysisCASAVA数据分析MSRBaseSpaceFORRESEARCHUSEONLYPart#④①①③④②②双端标签文库①FlowCell)结合的区域与流动槽(和Read2测序引物结合的区域Read1②插入片段③④标签序列区域(Index)片段两端加上能够与DNA文库制备的目的是在需要测序的测序仪配合的接头序列(Multiplexed,SR,PE)FORRESEARCHUSEONLYPart#-RapidHiSeqSequencingHiScanSQ测序GAIIxMiSeqNextSeqICS/RTADataAnalysisCASAVA数据分析MSRBaseSpaceFORRESEARCHUSEONLYPart#??是什么流动槽(FlowCell)ursonaflowcell簇生成在流动槽(flowcell)plementarytolibraryadapters每条通道中都随机植入了能与文库接头互补结合的大量短DN***段FORRESEARCHUSEONLYPart##&Extend片段杂交与延伸AdaptersequenceSingleDNAlibrariesare接头序hybridizedtoprimerlawn文库与流变性后单链DNA动槽上的引物杂交Boundlibrariesarethenextendedbypolymerases合成酶的作用随后在DNA文库进下,结合上的DNA行延伸Surfaceofflowcellcoatedwithalawnofoligopairs流动槽表面随机植入了大量引物链extens