文档介绍：高效液相色谱法测定余甘子药材中槲皮素的含量
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【摘要】目的建立余甘子药材中槲皮素含量测定的方法。方法HPLC法测定槲皮素含量。色谱柱为SHIMADZU VP-ODS (5 μm ,250 mm× mm),保护柱为大连中汇达C18,流动相:甲醇-%磷酸溶液( g·L-1庚烷磺酸钠)(50∶50), ml·min-1,检测波长373 nm。~ μg范围内与色谱峰的峰面积呈良好的线性关系(r= 7),% ,%(n= 6)。结论该方法操作简便、快速,有良好的精密度和准确性,可用于余甘子药材的质量控制。
【关键词】余甘子; 槲皮素; 高效液相色谱法
AbstractObjectiveTo establish a method for determination of the content of quercetin in Phyllanthus emblica L..Methods Quercetin was quantitatively determined by high performance liquid chromagraphy(HPLC).With SHIMADZU VP-ODS (5μm, mm×250 mm) column,a mixture of methanol and % H3PO4(with g
·L-1 C7H15SO3Na)(50∶50) was used as mobile phase,the flow rate was 1 ml·min-1 and UV detection wavelength was at 373 nm. ResultsThe linear relationship was good within the range of ~ μg(r =).The average recovery of Chinese medicinal plant Phyllanthus was % and the relative standard deviation was below % (n = 6).ConclusionThe method is fast,simple,accurate and reproducible and can be used for the quality control of Phyllanthus emblica L.
Key wordsPhyllanthus emblica L.; Quercetin; HPLC
余甘子为大戟科叶下珠属(Phyllanthus)植物余甘子Phyllanthus emblica 。《云南省药品标准》《藏药标准》《中国药典》均收载。别称滇橄榄、橄榄、油甘子、望果;藏药称“久如拉”,傣药称“麻项帮”;古印度梵语称“庵摩勒”。在云南地区常作橄榄使用,还是藏族习用药材。味酸、甘、涩,主要用于治疗胃痛、肠炎、黄疸型肝炎、淋巴结核、高血压病等。研究表明余甘子具有抗氧