文档介绍：Thiophilic Adsorption
Chromatography
II William Hutchens
1. Introduction
Thiophilic adsorption is useful for the purification of immuno-
globulins under mild conditions (., see ref. I). Although there are
several established procedures for the purification of immunoglobu-
lins (Z-5), thiophilic adsorption appears thus far to be unique in its
capacity to adsorb three major classes of immunoglobulins (and their
subclasses) (68). Furthermore, in contrast to other affinity purifica-
tion methods (., see refs. 3,4), recovery of the adsorbed (purified)
immunoglobulins from the thiophilic adsorption matrix is -
plished efficiently at neutral pH, without the need for perturbation of
protein structure (I). The most important utility of thiophilic adsorp-
tion is perhaps its use for the selective depletion of immunoglobulins
plex biological fluids (., calf serum and hybridoma cul-
ture media, colostrum and milk) (6,7,9). This latter development has
been particularly useful with hybridomacell culture applications (9,10),
and in the investigation of milk-immunoglobulin function during early
periods of human infant nutrition (6,7).
Thiophilic adsorption actually describes the affinity of proteins for
a specific set of sulfurcontaining (., thioether-sulfone) immobilized
ligands, which is observed in the presence of certain salts. Since the
first demonstration of this adsorption phenomenon (II), it has been
further developed and used primarily for the selective adsorption of
From: Methods m Molecular B/ology, Vol 17. Practical Protem Chromatography
Edlted by. A. Kenney and S. Fowell Copynght 0 1992 The Humana Press Inc , Totowa, NJ
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2 Hutchens
immunoglobulins (., seerefs. 1,6-8). Even though thiophilic adsorp-
tion chromatography, like hydrophobic interaction chromatography,
is a salt-promoted adsorption process, hydrophobic proteins, such as
serum albumin, are not thiophilic (10). The mechanism