文档介绍：摘要蓖麻是一种综合利用价值很高的植物,具有广阔的商业开发前景。其栽培历史悠久,是世界十大油料作物之一。但蓖麻营养生长过于茂盛,增加了很多不必要的生产成本。而矮秆蓖麻在同等条件下具有节水、抗早、抗倒伏的优点,能够提高产量、且易于栽培,因而蓖麻矮化育种、矮化基因的发掘已成为蓖麻育种的一个新的热点。本研究以始花期新鲜的蓖麻茎秆为试验试材, 技术筛选与矮化相关的基因。主要研究成果如下: 试验利用256对引物组合进行了选择性扩增,共得到300条差异基因,通过回收、克隆得到了130条目的基因片段,并对其进行了序列测定,通过BlastX 进行了同源性检索。其中3l条基因所表达的蛋白与己知蛋白具有较高的同源性, 其余为未知蛋白,包括预测蛋白、假想蛋白及未命名蛋白。同源蛋白功能主要有: 参与种子萌发、木质部形成及程序化死亡;参与糖酵解途径及遗传物质代谢调; 参与葡糖木聚糖(GX)以及纤维素生物合成等。确定了最适的蓖麻茎秆cDNA. AFLP分析体系,为蓖麻株高、株型等相关基因的研究奠了定基础。,可以便捷的将不同的生理结构或不同植株株型的差异显示片段找出来。本研究还发现了很多与矮化相关的未知功能基因,可能对进一步探索蓖麻矮化机制有重要意义,同时也说明蓖麻中仍有很多优异的基因资源有待发掘。关键词:蓖麻;矮秆;;分子标记 Separartion ofDwarf-related Genes ofCastor byUsing eDNA-AFLP Technology Abstract Castor is ahigh utilization value has broad prosplects mercial long cultivatehistory itis one oftheworld’Stop vegetative growth iS too lush SOthat add a10tofunnecessary production dwarf castor hastheadvantage ofwater-bating adrought and lodging resistance under tllesame iSeasy tocultivate and also tmprove introduction the breeding dwarf and the dwarf gene excavatlon has e anew study usedthefreshstem on thebeginning oftheflowering ofcastor as thegenes related todwarf with main results are asfollows: 256 binations were used for selectiveamplification,and 300 transcript derived fragments fTDFs)were selected fortheirdifferentialexpression,of which 30target genes were function oftargetgenes was identifiedby BlastXhomology genes expressed highhomology function 谢th aknown rest are unknown contains predicted protein, hypothetical proteinand unnamed protein。Other main functions ofhomologous proteins are asfollow:Function ofinvolving in seedgermination,xylem formation and programmed ofinvolving intheglycolytic pathway and metabolic transfer ic ofinvolving inglucose