文档介绍：长春师范学院
硕士学位论文
酸枣仁化学成分的分离提取及抗氧化活性研究
姓名:白鹤龙
申请学位级别:硕士
专业:分析化学
指导教师:刘春明
2010-06
摘要

酸枣仁为鼠李科枣属植物酸枣[ziziphphus jujubaMill var. spinosa (Bunge) Hu
ex chou]的干燥成熟种仁,其主要有效成分为黄酮类化合物,具有补肝,宁
心,敛汗,生津等作用。因此,对其化学成分进行系统的研究,具有潜在的意义,
为酸枣仁的进一步开发提供了一种分离纯化方法,为酸枣仁的开发利用奠定基
础。
,比较了回流提取法和超声
提取法对酸枣仁总黄酮含量的影响规律,采用分光光度法对酸枣仁总黄酮含量
进行了分析测定,确定了其最佳提取工艺。
,以
乙酸乙酯:正丁醇:水(3:2:5 v/v/v)作为溶剂系统,首次从酸枣仁粗提物
中分离得到:酸枣仁黄酮碳苷(spinosin)、6’’’- 阿魏酰酸枣仁黄酮碳苷
(6’’’-feruloylspinosin)及其衍生物,利用 ESI-MS 技术并参照文献确定了化合
物的结构,采用高效液相色谱法,对酸枣仁中黄酮碳苷、6’’’-阿魏酰酸枣仁黄
酮碳苷及其衍生物的含量进行测定。
、邻苯三酚自氧体系和双氧水体系分别对酸枣仁不
-
同浓度提取液和不同溶剂提取液清除·OH、O2 ·和H2O2的能力与Vc进行了比较研
- -
究。结果表明酸枣仁黄酮类成分对·OH、O2 ·和H2O2具有一定的清除作用,对O2 ·的
清除作用明显,清除率最高达到64%,是同浓度的Vc清除率的三倍。

关键词:酸枣仁;黄酮;高速逆流色谱;抗氧化活性

III
Abstract

Zizyphi spinosi semen (ZSS) is the seed of Ziziphus jujube Mill. var. spinosa
(Bunge) Hu ex . Chou (Rhamnaceae), which distributes to the heart、liver and
hall bladder total flavonoids of the ZSS extract are the main
pharmacological pounds. Therefore, It is potential significance for further
development of studing systematicly on the position of ZSS. And it
provides a new method of isolating and purifying for the further development of
, it builds up foundation for development.
Firstly,we research the process of extraction of flavonoids from ZSS, pare
the content of total flavonoids extracted by reflux extraction and ultrasonic extraction,
determined the content of total flavonoids by spectrophotometry; we obtain the best
extraction process.
Secondly,we develop preparative to isolate pounds from
ZSS. The solvent system posed of ethyl acetate:n-butyl alcohol:water(3:2:5
v/v/v ), we can obtain three pounds first time: spinosin 、
6’’’-feruloylspinosin and derivative, The structures pounds are identified by
ESI-MS and papers, we develop a method for quantitative determination of spinosin