文档介绍：The DNA Chip and The Process of DNA Chip
Contents
Introduction
DNA Chip 개요
DNA Chip 분석시스템
DNA Chip의처리
상용 tool의소개
Microarray Analysis System의개발
Conclusion
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Introduction
HGP(Human Genome Project) & Celera Genomics
$250 million, over 1100 scientists
Functional genomics : 단백질들의기능을부여하는작업
Structural genomics : genome의구조에대한연구
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DNA Chip 개요(1/3)
필요성
현재까지 28종의 gene 유전자배열이밝혀짐(/mdb/)
유전자암호해독기술(DNA sequencing)의발달
밝혀지는생명체 genome의급속한증가
한종의 genome에대한엄청난데이타양
(인간: 3000mbp - 30 억개의염기, 10만개의유전정보)
post genome시대에는새로운기술의개발이요구
DNA Chip의이용
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DNA Chip 개요(2/3)
정의
어떤슬라이드박편에여러가지종류의 DNA염기가닥(sample clone)을행렬형태로심어놓은것
sample gene PCR로증폭 robot printing(arrayer)
유전자발현의비교분석에용이
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Microarray제작과정
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DNA Chip 개요(3/3)
종류
spot에놓이는종류에따른분류
cDNA chip : 500bp이상의유전자
oligonucleotide chip : 15 ~ 25개의염기들로이루어진 oligonucleotide(분자단위의당)
제작방법에따른분류
pin microarray : pin을이용한 micro dotting(surface contact), 1995년 stanford 대학에서개발
Inkjet : Inkjet원리를이용한 micro dropping
Photolithography : Photolithography를이용한 oligonucleotide직접합성
Electronic array : 전기를이용한 oligonucleotide addressing
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Microarray 제작방법에따른분류
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DNA Chip 분석시스템(1/5)
Three Major
Keeping track of the information generated at the stages of DNA chip production and hybridization experiments
Processing microarray images to obtain the quantified gene expression values from the arrays
Mining the information from the gene expression data
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DNA Chip 분석시스템(2/5)
Data Processing Stage
Array Fabrication
Array Image Analysis
automatic spot finding and adjustment
automatic data extraction and quantification
pair-wise analysis
links to databases
Array Data Analysis
time series visualization
detailed spot information for any selected gene
identification of genes with salient expression patters
clustering
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