文档介绍：Progress in Nuclear ic Resonance Spectroscopy 32 (1998) 287–387
The use of NMR methods for conformational studies of nucleic acids
Sybren S. Wijmenga*, Bernd . van Buuren
Umea˚ University, Department of Medical Biochemistry and Biophysics, S 901 87 Umea˚, Sweden
Received 10 July 1997
Contents
1. Introduction .................................288
2. RNA and DNA synthesis and purification . . . ......................290
3. Nomenclature .................................291
4. Distances .................................291
. Overview of short distances and their general characteristics . . . . . ...........292
. Overview of structurally important intra-nucleotide distances . . . . . ...........294
. Overview of structurally important sequential and cross-strand distances ...........295
. Derivation of distances from NOESY spectra and structure characterization using distances . . . 295
. Conclusion .................................304
5. J-couplings .................................305
1 1
. JHC- and JCC-couplings . .............................307
. Overview of J-couplings in the bases . . . ......................307
. Ribose sugar .................................307
. Determination of the b torsion angle. . . . ......................310
. Determination of the e torsion angle . . . . ......................312
. Torsion angle g and H5Ј and H5ЈЈ stereo specific assignment. . . . . ...........314
3
. x torison angle and JHC sugar to base . . . ......................316
. Measurement of homo- and heteronuclear J-coupling constants . . . . ...........316
. Determination of J-couplings from the shape of the signal . . . ...........316
. Determination of J-couplings from patterns . . . . . ...........318
. Homonuclear . . ......................318
. Heteronuclear . . ......................319
. Determination of JHP- and JCP-couplin