文档介绍：Proteomic
复旦大学生命科学学院
吴超群
10/27/2005 Chaoqun Wu, Fudan University 1
DefiningDefining ProteomicsProteomics
Branch of discovery science focusing on proteins
In 1994 defined as “plete set of proteins that is
expressed and modified following expression by the
entire genome in the lifetime of a cell”. If we look at an
organism it means that we are looking at the proteome of
3 trillion of cells and ~1000 different cell types with
different protein profiles.
Can be more specific such as plement of
proteins expressed by a cell at any one time.
Today proteomics is a scientific discipline that will bridge
the gap between our understanding of genome
sequences and cellular behavior.
10/27/2005 Chaoqun Wu, Fudan University 2
GenomicsGenomics andand ProteomicsProteomics aa newnew
fieldfield withwith aa newnew vocabularyvocabulary
--OmicsOmics:: DNA Genome
meansmeans areaarea
ofof researchresearch
RNA Transcriptome
proteins Proteome
Metabolome
Metabolites
Protein-protein, Protein-DNA, Interactome
Protein-RNA interactions
10/27/2005 Chaoqun Wu, Fudan University 3
TheThe ““omicsomics”” nomenclaturenomenclature……
Genomics DNA (Gene)
Transcription
Transcriptomics RNA
Functional Translation
Genomics
Proteomics PROTEIN
Enzymatic
reaction
Metabolomics METABOLITE
10/27/2005 Chaoqun Wu, Fudan University 4
WhyWhy studystudy proteinprotein expression?expression?
Nucleus Cytosol
Inactive mRNA
RNA
Degradation
control
Primary
DNA RNA mRNA mRNA
transcript
Translation control
RNA
Modified
Transport protein
RNA protein
Transcriptional Processing control
control
control Post-translational
control
10/27/2005 Chaoqun Wu, Fudan University 5
TheThe proteomeproteome
–– plement ofof aa gengenomeome
Variable
– In different cell and tissue types in anism
– In different growth and developmental stages of
organism
Dynamic
– Depends on response of genome to environmental
factor