文档介绍：Part VI.
Structural
Proteomics
10/27/2005 Chaoqun Wu, Fudan University 1
StructuralStructural proteomicsproteomics
StructuralStructural proteomicsproteomics hashas thethe goalgoal ofof
obtainingobtaining useful,useful, three-dimensionalthree-dimensional
modelsmodels ofof allall proteinsproteins byby bina-
tiontion ofof experimentalexperimental structurestructure
determinationdetermination parative modelmodel
.
10/27/2005 Chaoqun Wu, Fudan University 2
TheThe experimentalexperimental (left)(left) putational (right)(right)
hierarchieshierarchies willwill increasinglyincreasingly e codependentcodependent asas thethe
munity modelsmodels greatergreater plexity.
10/27/2005 Chaoqun Wu, Fudan University 3
GlobalGlobal structuralstructural genomicsgenomics effortsefforts willwill bebe majormajor playersplayers inin
pleting thethe proteinprotein familyfamily andand foldfold .
10/27/2005 Chaoqun Wu, Fudan University 4
10/27/2005 Chaoqun Wu, Fudan University 5
It is feasible to determine at least 10,000
protein structures within the next five years.
No new discoveries or methods are needed
for this task.
The average cost per structure is expected
to decrease significantly from $200,000 per
structure to perhaps less than $20,000 per
structure.
10/27/2005 Chaoqun Wu, Fudan University 6
Large-scale protein structure modeling.
A small sample of the 1,parative models calculated
for the proteins in the yeast genome is displayed over an
image of a yeast cell
10/27/2005 Chaoqun Wu, Fudan University 7
Experimental structure determination
蛋白质的
体外翻译
蛋白质的生选择性蛋白质
物活性检测分子的突变
10/27/2005 Chaoqun Wu, Fudan University 8
1. Target selection (Grouping)
TheThe targetstargets forfor structurestructure determinationdetermination willwill bebe
. IndividualIndividual domainsdomains ratherrather thanthan multi-domainmulti-domain
proteinsproteins (Chris(Chris Sander,Sander, MilleniumMilleniu