文档介绍:Chapter 7 Microbial Growth and Growth control
Overview of Cell Growth
Population Growth
Measurement of Growth
Continuous Culture: The Chemostat
Effect of Environment on Growth
Growth Control
Viral Control
Fungal Control
Overview of microbial growth
The bacterial cell is a synthetic machine that is able to duplicate itself. The synthetic processes of bacterial cell growth involve as many as 2000 chemical reactions of a wide variety of types. Some of these reactions involve energy transformations. Other reactions involve biosynthesis of small molecules-the building, blocks of macromolecules-as well as the various cofactors and coenzymes needed for enzymatic reactions.
Binary Fission
6 – 1. Microbial Nutrition
Nutrient requirements
Nutritional types of anisms
Uptake of Nutrients by the Cell
Culture Medium
Isolation of Pure Cultures
anisms require about ten elements in large quantities, because they are used to construct carbohydrates, lipids, proteins, and nucleic acids. Several other elements are needed in very small amounts and are parts of enzymes and cofactors.
Concepts:
Nutrient requirements
Macronutrients
95% or more of cell dry weight is made up of a few major elements: carbon, oxygen, hydrogen, nitrogen, sulfur, phosphorus, potassium, calcium, magnesium and iron.
The first six ( C, H, O, N, P and S) ponents of carbonhadrates, lipids, proteins and nucleic acids
Trace Elements
Microbes require very small amounts of other mineral elements, such as iron, copper, molybdenum, and zinc; these are referred to as trace elements. Most are essential for activity of certain enzymes, usually as cofactors.
Growth Factors
Amino acids are needed for protein synthesis,
purines and pyrimidines for nucleic acid synthesis.
Vitamins are anic molecules that usually make up all or part enzyme cofactors, and only very small amounts are required for growth.
(1)amino acids, (2) purines and pyrimidines, (3) vitamins
Major nutri