文档介绍：Molecular Biologists Guide to Proteomics
Christopher Kvaal
Montana State University-Bozeman
Source: Molecular Biologist Guide to Proteomics, Graves, . and Haystead, ., Micro Mol Biol Rev. Mar 2002 pp 39-63
Topics of Discussion
Review
Introduction to Proteomics
Technology of Proteomics
Applications of Proteomics
Review
Bioinformatics
Storing, Organizing, and Analyzing Sequence Data
Functional Genomics
Contextual Use of Bioinformatic Data
. DNA Array or SAGE
Proteomics
Why do we have these new disciplines?
To much information
What do we do with all the sequence data?
45 anism genomes have been sequenced and 170 more are in progress
5 eukaryotes have pleted
haromyces cerevisiae
haromyces pombe
Arabodopsis thaliana
Caenorhabditis elegans
Drosophilia melanogaster
Rice, Mouse and Human are nearly done
2/3 of all genes “identified” have no known function
Move over Genome……on to Proteomics
If the genome is the blueprint of anism---who reads it?
The proteome is all the proteins that are called for by the genome
At this point puter algorithm can do this
puter can decode all 6 reading frames of anism
puter pare these.. But then what?
Genomic DNA
Structure
Regulation
puters cannot determine which of these 3 roles DNA play solely based on sequence…(although we would all like to believe they can)
Introduction to Proteomics
Definitions
1. Classical - restricted to large scale analysis of gene products involving only proteins
2. Inclusive - combination of protein studies with analyses that have ponents such as mRNA, genomics, and yeast two-hybrid
Don’t forget that the proteome is dynamic, changing to reflect the environment that the cell is in
1 gene = 1protein?
1 gene is no longer equal to one protein
In fact, the definition of a gene is debatable..(ORF, promoter, pseudogene, gene product, etc)
1 gene=how many proteins
Why Proteomics?
Why Proteomics?
Annotation of genomes, . functional annotation
Genome + proteome = annotation
Protein Function
Pr