文档介绍：Proteomics to study genes and genomes Nature , Jan 2000
指導教授: 唐世杰博士
講者: 蔡一麟、周仁祥、陳宏偉
Proteomics (蛋白質體學)
1970s Researchers started to build databases of proteins using the newly developed technique of two-dimensional
gel electrophoresis.
1990s Biological mass spectrometry emerged as a powerful analytical method that removed most of the limitations of protein analysis. It coupled with the availability of the entire human coding sequence in public databases.
Proteomics is the large-scale study of proteins, usually by biochemical methods.
Why is proteomics necessary? 1. Complete sequences of genomes is not sufficient to elucidate biological function. 2. Proteomics directly contributes to drug development as almost all drugs are directed against proteins. 3. The existence of an open reading frame (ORF) in genomic data does not necessarily imply the existence of a functional gene. 4. Modifications of the proteins that are not apparent from the DNA sequence.
Biochemical Context of Genomics and Proteomics
DNA Genome
“Genomics”
mRNA
Proteins Proteome
“Proteomics”
Bioinformatics
Proteomics can be divided into three main areas:
(1) Protein micro-characterization for large-scale
identification of proteins and their post-translational modifications.
(2) “Differential display”proteomics parison
of protein levels with potential application in
a wide range of diseases.
(3) Studies of protein–protein interactions using
techniques such as mass spectrometry or the
yeast two-hybrid system.
The key technique involves in proteomics.
spectrometry
-dimensional gel electrophoresis
two-hybrid system
A strategy for mass spectrometric identification of
proteins and post-translational modifications.(PDGF)
Cell lysate from Escherichia coli analysed by mass.