文档介绍：THE CHARACTERIZATION OF ANTIZYME IN DEVELOPING Xenopus laevis EMBRYOS
Alexandra Silveira and Charles Toth Department of Biology
Providence College, Providence, RI
Protein Degradation
Cell cycle and proliferation
Differentiation and development
Stress response
Genesis of works
Modulation of cell surface receptors
Regulation of immune response
Apoptosis
Transcriptional regulation
Pathogenesis
Cancer, cystic fibrosis, neurodegenerative diseases
Charles Toth, Providence College
Protein degradation by the 26S proteasome
t/bcbp/molbiochem/MBWeb/mb2/part1/
Protein degradation by the 26S proteasome
ODC degradation – a unique protein degradation pathway
John Mitchell, University of Northern Iowa
John Mitchell, University of Northern Iowa
ODC and Polyamine Biosynthesis
/mitchellab/
Polyamines
pounds found bound to RNA and DNA in cells
High levels are toxic
Necessary for cell growth and survival
gate currents of ion channels
have effects on chromatin condensation and transcriptional regulation
neutralize negative charges of RNA and DNA
regulate the levels of AZ
provide a necessary postranslational modification to eIF-5a protein
/zoofysiol/Cellprolif/Research/
AZ Functions
ODC degradation
Anti-proliferative effect on cells
Blocks uptake of additional polyamines when polyamine levels are too high
High levels of polyamines in turn regulate AZ through a ribosomal frameshift
/mitchellab/
Ribosomal Frame-Shifting
High levels of polyamines cause a ribosomal frame shift that produces an active form of AZ.
Without the ribosomal shift a truncated and inactive AZ is formed.
Why study antizyme?
Studying the unique ODC degradation pathway can lead to a better understanding of
ubiquitin mediated protein degradation
the many roles of polyamines in the cell
AZ is a member of an ancient gene family
AZ1 and AZ2 widely expressed and involved in ODC degradation
AZ3 is testis specific