文档介绍：Hong Xu E-mail: xuhong1685@ Tel: 86971913 (O) 191352118 (QQ or 微信)第五讲蛋白质翻译后修饰 Post translational modification 各物种基因数量比较细菌: ~4400 酵母: ~5800 果蝇: ~14000 小鼠: ~22000 人: ~25000 Genotype Phenotype 橘生淮南则为橘生于淮北则为枳叶徒相似其实味不同所以然者何水土异也 4 genomic DNA biological system mRNA functional protein protein products sequencing expression profiling structural determination protein linkage maps(catalog) quantitative protein profiling protein linkage maps(dynamic) activity profiling post-translational modification analysis inferred activity data integration data integration subcellular localization technology emerging prototype mature lncRNA microRNA 中心法则5 Molecular Biology of the Cell 有序表观遗传学( ics ) —— DNA methylation , Histone modification, RNA interference 复制( replication ) Protein -> Protein, Protein -> DNA, DNA -> Protein 蛋白质修饰( protein modification ) 8 Outlines ( 3W1H ) ? What is Post-translational modification ? ? What types of Post-translational modifications are exist? ? Wh at is the biological meanings of protein post- translational modifications? ? How to detect the Post-translational modification? What is Post-translational modification (PTM)? Protein modifications - different types, including co-translational and post- translational - inteins Co- and Post-Translational Modifications ? Co-translational modifications - occurs during protein synthesis ? N-terminal processing ? N- glycosylation ? Partial proteolysis Protein modification ? Post-translational modifications - occurs after protein synthesis ? phosphorylation ? methylation ? O- glycosylation