文档介绍：Revised by Petrel at 2021
常用蛋白酶切割位点
常用融合蛋白切割位点
PROTEASE
COMPANY
Protease Capture
Thrombin （凝血酶）
Leu-Val-Pro-Arg▼Gly-Ser
L-V-P-R▼G-S
Amersham-Biosciences, Novagen,
SIGMA, Roche
Benzamidine-Agarose
Factor Xa （Xa因子）
Ile-Glu/Asp-Gly-Arg▼?
I-E/D-G-R▼
Amersham-Biosciences,
New England Biolabs,
Roche
Benzamidine-Agarose
Enterokinase （肠激酶）
Asp-Asp-Asp-Asp-Lys▼
D-D-D-D-K▼
New England Biolabs,
Novagen, Roche
Trypsin Inhibitor-Agarose
TEV protease （烟草蚀纹病毒蛋白酶）
Glu-Asn-Leu-Tyr-Phe-Gln▼Gly
E-N-L-Y–F-Q▼G
Invitrogen – Life Technologies
Ni-NTA (6His recomb. TEV)
PreScission （PreScission 蛋白酶）
Leu-Glu-Val-Leu-Phe-Gln▼Gly-Pro
L-E-V-L-F-Q▼G-P
Amersham-Biosciences
GSTrap for GST fusion enzyme
TAGZyme （标记酶）
His-tag removal by Exoproteolytic Digestion
Qiagen
Ni-NTA (6His recomb. enzyme)
Intein Site （内含肽）
dithiothreitol cleavage（二硫苏糖醇清除）
New England Biolabs
DTT elimination by dialysis?（透析）
HRV 3C Protease（3c蛋白酶）
Leu-Glu-Val-Leu-Phe-Gln▼Gly-Pro
L-E-V-L-F-Q▼G-P
Novagen
Ni-NTA (6His recomb. enzyme)
SUMO Protease
recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO
Invitrogen – Life Tech