文档介绍：HPLC 测定咳喘宁颗粒中盐酸麻黄碱的含量孙杰谷学新*任占军赵新颖(首都师范大学化学系 100037) 摘要: 目的利用高效液相色谱法测定咳喘宁颗粒中盐酸麻黄碱的含量。方法采用 EconosphereC 18柱(5μ m, × 250mm) , 流动相为乙腈— % 三乙胺(加磷酸调 pH 为 )(5 : 95) ,检测波长: 207nm ,流速: L· min -1, 柱温: 30℃。结果盐酸麻黄碱在 ~ μ g 范围内线性关系良好(r=) , 回收率为 % , RSD 为 %(n=5) 。结论该方法简便可靠,快速,重现性好,可用于咳喘宁颗粒的质量控制。关键词:麻黄;盐酸麻黄碱; 咳喘宁颗粒 RP - HPLC Determination of Ephedrine in kechuanning keli SUN Jie , GU Xu- xin *, REN Zhan-jun , ZHAO Xing-ying , ( chemistry department in capital normal university 10037) ABSTRACT: OBJECTIVE A RP-HPLC method was used for the determination of ephedrine in kechuanning keli. METHODS The chromatographoric condition were: EconosphereC 18 (5μ m, × 250mm) column , and the mobile phase was consisted of % triethylamine in water — acetonitrile (95 : 5) (adjusted to pH= with phosphoric acid), 207nm as detected wavelengths, the flow rate was -1. RESULTS The content of kechuanning had good linear relationship in ~ μg (r=).The average recovery was %, RSD 为 %(n=5). CONCLUSION The method is accurate ,reliable and can be used for the quality control of kechuanning product. KEY WORDS: ephedrine ; kechuanning 咳喘宁颗粒由部颁标准[1] 麻黄、紫菀、苦杏仁、百部、甘草等药材组成。其中麻黄为方中君药, 麻黄的主要活性成分为麻黄碱, 麻黄碱具有解热、抗病毒、松弛支气管平滑肌等作用[2]。通过测定咳喘宁颗粒中盐酸麻黄碱的含量, 可以作为控制该产品质量的一个重要指标。文献已报道的麻黄碱含量测定方法有滴定法[3] 、薄层扫描法[4] 、高效液相色谱法[5] 等。本实验采用高效液相法测定咳喘宁颗粒中麻黄碱的含量, 操作方