文档介绍：Prescott−Harley−Klein: XI. Food and Industrial 42. Industrial Microbiology © The McGraw−Hill
Microbiology, Fifth Edition Microbiology and panies, 2002
1000 Chapter 42 Industrial Microbiology and Biotechnology
Table Methods Used to Preserve Cultures of Interest for Industrial Microbiology and Biotechnology
ments
Periodic transfer Variables of periodic transfer to new media include transfer frequency, medium used, and holding
temperature; this can lead to increased mutation rates and production of variants
Mineral oil slant A stock culture is grown on a slant and covered with sterilized mineral oil; the slant can be stored at
refrigerator temperature
Minimal medium, distilled water, or water agar Washed cultures are stored under refrigeration; these cultures can be viable for 3 to 5 months or longer
Freezing in growth media Not reliable; can result in damage to microbial structures; with some anisms, however, this can
be a useful means of culture maintenance
Drying Cultures are dried on sterile soil (soil stocks), on sterile filter paper disks, or in gelatin drops; these can be
stored in a ator at refrigeration temperature, or frozen to improve viability
Freeze-drying (lyophilization) Water is removed by sublimation, in the presence of a cryoprotective agent; sealing in an ampule can lead
to long-term viability, with 30 years having been re