交易模型编写教程.pdf.pdf

文档介绍：P art1To 900ml DDH2O in2 litre shake flask add (NH4)2SO4 = 50 Mm add first KH2PO4 add second 1M MgSO4 stock solution 1M CaCl2 stock solution 30 Mm FeSO4/50mM EDTA stock solution() 50 Mm CuSO4 stock solution A utoclave ’P art2To 400ml DDH2O add KH2PO4 NaH2PO4 A djust pH to pH with 10N NaHO,adjust volume to 500 ml with DDH2O and autoclave in 100ml fractions in 250 ml flasks P art3M ake up 500ml 5%NaCO3(anhydrous) and autoclave HOW TO GROW THE BUGS A dd 1*100ml aliquot of part 2 to 1*900ml aliquot of part add 8ml of part add 10ml of 3days old culture to new media and incubate on rotary shaker (100~150RPM)at 30 ℃.The bugs should grow up just fine in three to four days.