文档介绍：温肾调经颗粒质量标准研究
李红英于得才杨丽闫建坤
【摘要】 目的建立温肾调经感咳颗粒的质量标准。方法采用TLC法对 处方中的肉欢蓉、菟丝子、茜草进行定性鉴别;采用HPLC法测定阿魏酸的 含量。色谱柱为Kromasil C18柱(250mmX4. 6mm, 5 u m);流动相为甲醇-5% 醋酸(20:80);流速为1. Oml/min;检测波长323nmo结果进样浓度在2. 50〜 20. 00 u g/ml范围内线性关系良好(r=0. 9999);平均回收率98. 54%, RSD为 %(n=5)o结论本方法操作简单，准确可靠，重复性好，可作为温肾调 经颗粒的质量控制方法。
［关键词】 温肾调经颗粒；阿魏酸;薄层色谱；高效液相色谱
Study on quality control standarad of Wenshen Tiaojing Granules
LI Hong-ying, YU De~cai, YANG Li , et al. Shandong Wego Medicine Co., Ltd., Weihai 264209, China
[Abstract]0bjectiveThe quality Control Standarad of Wenshen
Tiaojing Granules were established. MethodsA TLC method was used for
the qualitative identification of Cistanches Herba and Rubiae Radix Et Rhizoma and Cuscutae Semen in the formula. The content of Ferulic Acid was determined by a HPLC method with a Kromasil C18(250mmX 4. 6mm, 5 u m)column and a mobile phase consisted of Methanol- 5%Acetic Acid(20: 80).The flow rate was . The detection wavelength was 323nm. ResultsThe linear relation was good within the range of 〜20. 00 u g/ml(r=0. 9999)and average recovery was %, RSD1. 22%(n=5). ConclusionThe method was
simple, accurate, reliable and reproducible. It could be used in the quality control method of Wenshen Tiaojing Granules.
[Key words]Wenshen Tiaojing Granules:Ferulic Acid;TLC;HPLC
温肾调经颗粒主要由当归、杜仲、菟丝子、肉疝蓉、茜草等药物组 成，其处方来源于临床验方，具有温肾养血，利温化浊，祛瘀调经之功效, 临床上用于肾阳不足，冲任血虚，湿