文档介绍：2
3
2
2
2
2
2
2 2
2 2 2 2
2
2
2
2
2 2 2
2 2 2
安徽农业科学,Journal of Anhui Agri. Sci. 2007 ,35 (18) :5358 - 5360 责任编辑孙红忠责任校对李洪
2
ERIC PCR 技术特点及其应用 2 2
2
李犹平倪学勤(四川农业大学动物科技学院,四川雅安 625014)
, 2
2
摘要 ERIC 序列是近年来发现的存在于原核生物基因组中一类短的重复序列。该序列在染色体上的分布和拷贝数具有种间的特异
性,根据序列中心高度保守的 44 bp 的 ERIC 核心序列设计反向引物,可扩增出反映细菌基因组结构特征的谱带。由于 ERIC PCR 快速简
便,图谱重复性好,能用于细菌分类鉴定、环境监测、污染治理以及人和动物肠道菌群结构研究等方面。
关键词 ERIC;IRU;ERIC PCR ;特点;应用
中图分类号 Q503 文献标识码 A 文章编号 0517 - 6611(2007) 18 - 05358 - 03
Characteristic and Application of the Enterobacterial Repetitive Intergenic Consensus PCR
LI You ping et al (College of Animal Science and Technology ,Sichuan Agricultural University ,Yaan ,Sichuan 625014)
Abstract Enterobacterial Repetitive Intergenic Consensus(ERIC) ,a kind of short duplication sequences ,is found to exist in the genome of prokaryotic or
ganism. Distribution and copy of the sequence in chromosome is interspecific specificity. According to the highly conservative core sequence ,44bp ,reverse
primer is designed. ERIC PCR can generate unique fingerprints to reflect the structural feature of bacterial strains ,and can identification species. ERIC
3
PCR technique can be used in the identification of bacterium ,environmental monitoring ,investigation intestinal flora of human beings and animals ,which is
2 2
rapid ,simple and repetitive.
Key words ERIC