文档介绍：柠檬桉叶油包合物的制备及其对小肠运动的影响指导老师: 作者: 摘要: 目的: 研究提取柠檬桉叶油的提取及其包合物制备工艺的最佳工艺条件、探究柠檬桉叶油包合物对动物小肠功能的影响。方法: 以挥发油产量和挥发油包合率为优选指标,运用正交实验法,对柠檬桉叶油的提取和包合的最佳工艺工艺参数进行优选; 分别采用离体家兔肠管和在体小鼠为试验对象, 观察柠檬桉叶油包合物对其运动功能的影响。结果: 提取柠檬桉叶油最佳工艺条件: 提取时间 , 料水比 1:1 和提取温度为 100 ℃; 超声波法包合柠檬桉叶挥发油的最佳工艺条件: 挥发油与β- 环糊精的比例、包合温度和包合时间分别为 1 ml: 10 g、 25℃和 h, 收得率为 % , 包合率可达到 % 。柠檬桉叶油包合物能增强小鼠小肠推进率, 浓度为 1× 10 -4 g/ml 以上的柠檬桉叶油包合物可增强家兔离体回肠的收缩力( P < )。结论:柠檬桉叶油包合物制备工艺简单可行, 能促进动物肠运动功能。关键词: 柠檬桉叶油;包合物;家兔;肠道运动 Preparation of Citriodora Oil Clathrate and the Effects of it onThesmall intestine Instructor: Professor Xia Jingming Author : Chen Dizhu (Chemical Science and Technology Institute , Zhanjiang Normal University, Zhanjiang 524048) Abstract: Objective : Study the best processing parameters of the e xtraction ofc itriodora o il and itsc lathrate and test the effects ofc itriodora o ilc lathrate on gastrointestinal motor function in mice and rabbit. Methods : Using t he yield and inclusion rate ofc itriodora oil as selection indicators, the best processing parameters of the e xtraction ofc itriodora o il and its c lathrate were studied by cross test . The effects of ofc itriodora oil on gastrointestinal motor functions were tested in mice in vivo and rabbits intestinal in vitro. Results: About t he best processing parameters of the extraction of citriodora oil, the best extraction time, proportion of material to water, and extraction temperture were h, 1:1 and 100 ℃ respectively. About t he best processing parameters of the extraction of citriodora oilc lathrate , the best proportion of citriodora oil to β-cyclodextrin ,i nclusion temperature and time were 1 ml: 10 g, 25℃ and h respectively , and the y ield and i nclusion rate were % and %. intestine push rate was increased by the certain dose of citriodora oilc lathrate in the mice in vivo. The i leum contractility was increased by citriodora oilc lathrate above the concentration of1× 10 -4 g/ml (P < ) in vitro. Conclusion: lemon euc