文档介绍:华中农业大学硕士学位论文
1 前言 ················································································································21
2 实验材料 ········································································································21
菌株、培养基及质粒 ··············································································21
主要实验仪器 ·························································································22
主要化学试剂 ·························································································22
主要溶液配方 ·························································································23
3 实验方法 ········································································································25
SH0165 菌株的培养条件·········································································25
外膜蛋白样品的制备··············································································25
蛋白定量··································································································26
双向电泳 (2D-PAGE) ········································································26
凝胶染色(银染)··················································································27
图像分析及酶解 ····················································································27
MALDI-TOF-MS 分析·············································································27
WESTERN-BLOT····················································································28
蛋白克隆表达及纯化··············································································28
引物的设计与合成 ··········································································28
引物的设计与合成···········································································28