文档介绍：Construction of a New Universal Plant
Overexpression Vector for cDNA Transformation

Dang-Quan ZHANG*, Zhen-Jun GU, Shun-Yang DENG, Shao-Gang FAN, Quan-Dong ZHU
Biotechnology Core Facilities / Key Lab of Non-wood Forest Products of State Forestry Administration,
Central South University of Forestry and Technology, Changsha 410004, . China
* E-mail: NWFP_zhangdq@

Abstract— The transformation of cDNA into plant is still not The majority of eukaryotic messages terminate in a poly(A) tail,
carried out directly by a universal transgenic vector, hence it is which is required for efficient translation and message
not convenient for the transgenic application of those function- stabilization [8]. The 3' untranslated region (UTR) of o
important cDNAs. Herein we introduced a rapid, cost-effective mosaic virus (TMV) genomic mRNA is required for promoting
PCR-based DNA synthesis method to construct a new universal efficient translation in plant [9], binate this sequence on
plant overexpression vector for the direct inserting of target to the vector to increase the stability of mRNA was translated.
cDNA during transgenic application. The new engineered The gene with the KDEL signal sequence was found 100-fold
transgenic vector was based on the pCAMBIA vector backbone, than the lack of KDEL signal sequence in transgene plant
which was inserted by f