文档介绍：Molecular Cloning, Expression and Enzymatic
Characterization of Inosine Monophosphate
Dehydrogenase from Bacillus amyloliquefaciens
Fei Wu, Xixian Xie, Jianming Shi, Qingyang Xu Ning Chen,
College of Bioengineering Key Laboratory of Industrial Microbiology Ministry of
Tianjin University of Science and Technology Education
Tianjin, China Tianjin, China
wufei_2009@ ******@tust.
Abstract—Inosine monophosphate dehydrogenase(IMPDH, GR600. and inserted into expression vector pET-His for high-
) is the rate-limiting enzyme for de nove guanosine level expression in Escherichia coli BL21(DE3). The binant
monophosphate synthesis. The IMPDH encoding gene guaB has IMPDH was purified essfully, a preliminary study of its
been clonded and sequenced from Bacillus amyloliquefaciens enzymatic Characterization have been carried on. The results
GR600, a overproduction-guanosine strain. A fragment could provide some theoretical basis for clarifying the
contained the stuctrural gene guaB encoding IMPDH from mechanism of accumulation of guanosine in Bacillus
GR600 was constructed into expression vector pET-His. The amyloliquefaciens GR600, and could give the foundation for
binant expression plamid was transformed into Escherichia the further producing bacteria selection by using ic
coil strain BL21(DE3), induced by IPTG and expressed. Th