文档介绍：T Cell Apoptosis Was Inhibited by Fas of Antisense Oligonucleotide
Shi-ying ZHENG, Dong JING, Jin-feng GE, Zhen-ya SHEN*
Department of Thoracocardiac Surgery, The First Affiliated Hospital of Suzhou
University ,Suzhou, Jiangsu, 215006, China
*To whom correspondence should be addressed: Tel: +86- 512-67780100;
Email:uuzyshen@
Abstract-We investigate the effect of specific tumor cells may escape immune clearance by a
antisense oligodeoxynucleotide (ASO DN) diversity of mechanisms including altering
inhibition of Fas expression on T cell apoptosis immune recognition or by modulation of the
induced by gastric carcinoma cell . Fas receptor cytotoxic response. Recent studies have shown
(Fas) and Fas ligand (FasL) expressed by the that the Fas receptor (Fas, APO-1/CD95) and its
gastric carcinoma cell line SGC-7901 and Jurkat T ligand (FasL, CD95L) as a major regulator of
cells were detected by flow cytometry (FCM) and both apoptosis and immune function has
the ability of FasL-inducing T cell apoptosis was provided insight into a range of attractive
tested by co-culture assay in vitro with SGC-7901 mechanisms by which tumors escape from
cells and Jurkat T cells. The Jurkat cells were immune clearance [1]. Cytotoxic T lymphocytes
transfected with Fas-ASODN using lipofectin, and (CTLs) play an important role in the immune
the effects of Fas-AS