文档介绍:Study on the effects of ecological engineering for ecological restoration on bacterial
community structure by molecular biological method in a eutrophic lake
FENG Sheng LI Dinglong MA Jianfeng
School of Environmental and Safety Engineering, Jiangsu Polytechnic University, Changzhou 213016, China;
Abstract:Study on munity structure and some molecular approaches have greatly
diversity in freshwater is important for exploiting the enhanced the knowledge of population structure
relationship of munity and its habitats, in natural munity [5]. With the
predicting and monitoring the ecological changing of
contaminated water habitat. The retrieval of 16SrDNA epifluorescence direct counting method (FDC)
genes directly from the environment has made it acquire the bacteria abundance exactly in water
possible to estimate the ic diversity of a column. The retrieval of 16SrDNA genes from
natural munity without preceding the environment directly make it possible to
cultivation steps, which reflect prehensively of estimate the ic diversity of natural
munity in the water samples in different munity without preceding
ecological restoration areas. The genomic DNA was
extracted from water, PCR amplification of this gene cultivation steps. 16SrRNA sequencing-based
fragments; DGGE was analyzed; the abundance of ic analysis and denaturing gradient
bacteria we